blog 8 transduction/transformation

Whathappens in bacterial transformation and transduction

Boyerand Cohen attempt combining ligated plasmids into the E.Coli. Using pastexperiments they use both bacterial and DNA and mix them by raising andlowering the temperature creating a heat shock. They then induce the bacteriato take plasmid DNA. Taking the transformed bacteria containing both type of resistance that could grow in presence of both antibiotics.This results consistently with the bacteria being transformed with a recombined plasmid(including tet and kan gene). Some bacteria is doubly transformed by religated versions of the original plasmids.